Features of Collagen Matrix Reconstructed with Proteodermatan sulfate from Pigskin Insoluble Collagen
نویسندگان
چکیده
منابع مشابه
The extraction of collagen protein from pigskin
As the collagen protein market increasingly grows, it becomes a priority to explore a economic, stable, and environmentally-friendly extraction method to realize the high-value transformation of pigskin. In this paper, the structure of collagen protein is introduced first. Then five methods, which extract collagen protein from pigskin or a kind of leather industry waste-chrome shavings, are rev...
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Alkaline treatment method was used to ext ract collagen. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism (CD) spectra revealed that the alkali-soluble pigskin collagen (ASPC) retained the polypeptide chains and triple helix conformat ion. The amino acid profiles showed no major deviation from the characteristic collagen composition. Both of the denat...
متن کاملProteodermatan sulfate isolated from pig skin.
The major proteoglycan of pig skin, a proteodermatan sulfate, has been isolated under mild conditions in the presence of protease inhibitors. After purification by ion exchange and gel chromatography, it has a Mr of 70 x 10(3) and it does not form aggregates with hyaluronic acid. It contains about 60% protein, one chain of dermatan sulfate, and several oligosaccharide chains. The dermatan sulfa...
متن کاملMatrix metalloproteinase interactions with collagen and elastin
Most abundant in the extracellular matrix are collagens, joined by elastin that confers elastic recoil to the lung, aorta, and skin. These fibrils are highly resistant to proteolysis but can succumb to a minority of the matrix metalloproteinases (MMPs). Considerable inroads to understanding how such MMPs move to the susceptible sites in collagen and then unwind the triple helix of collagen mono...
متن کاملModes of intermolecular cross-linking in mature insoluble collagen.
The structural unit of the collagen fibril is the tropocollagen molecule, a ropelike rodlet formed from three intertwined subunit strands of nearly equal molecular weight (I). Chromatographic analyses of denatured soluble collagens have indicated that the three subunit strands are all different, although two (designated al and a3) are very similar in composition (2). The third chain (012) is di...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369
DOI: 10.1080/00021369.1989.10869401